HBeAb Elisa Kit
HBeAb ELISA Kit
Enzyme-linked immunosorbent assay for the detection antibody against HBeAg in serum or plasma
SUMMARY AND PRINCIPLE OF THE TEST
The presence of antibody against hepatitis B viral e antigen is used as an indicator for (1) early HBs antigenemia before the peak of viral replication and (2) early convalescence when HBeAg has declined below detectable levels. It is also useful to confirm a seroconversion. The seroconversion from HBeAg positivity to anti-HBe positivity indicates a reduced level of infectious virus because virus replication has decreased.
Materials provided with the kits:
1.Microtiter Well coated with purified anti-HBe: 96 tests
2.Negative Control: One vial of 0. 5ml anti-HBe Negative Control.
3.Positive Control: One vial of 0. 5ml anti-HBe Positive Control.
4.Enzyme Conjugate: 6 ml containing HRP-conjugated-anti-HBe-HBeAg complex for 96 tests.
5.Wash Buffer Concentrate (20 x): 20 ml for 96 tests. The buffer should be diluted 20 times with distilled water before use.
6.Substrate Solution A: 6 ml HRP Substrate for 96 tests.
7.Substrate Solution B: 6 ml TMB Chromagen Substrate for 96 tests.
8.Stop Solution: One bottle of 6 ml 2N Sulfuric Acid.