HCV Elisa Kit
- Product description
HCV Ab ELISA Kit
Enzyme immunoassay for the detection of antibodies against Human Hepatitis Virus Type C (HCV)PRINCIPLE OF THE ASSAY
Multiple epitopes of HCV proteins(Core, NS3, NS4 and NS5) are bound to the microtiter wells. When antibodies to HCV are present in the test sample, they react with recombinant proteins and attach to the solid-phase. Non-reactive antibodies are removed with the wash buffer. Human IgGs bound to the antigen are reacted with anti-human IgG peroxidase conjugate and visualized by subsequent reactions with a chromogenic substrate. Positive sample generates a medium to dark blue color. No color or very pale blue color indicates a negative reaction. The intensity of the reaction is photometrically quantitated.
REAGENTS
Materials provided with the kits:Item
Description
96T
480T
1
Coating Plate
1
5
2
Negative Control
1ml
5ml
3
Positive Control
1ml
5ml
4
Sample Diluent
12ml
60ml
5
Enzyme Conjugate
12ml
60ml
6
Wash Buffer Concentrate (20x)
30ml
150ml
7
Substrate Solution A
6ml
30ml
8
Substrate Solution B
6ml
30ml
9
Stop Solution
6ml
30ml
10
Plastic Bag
1
5
11
Seal Paper
3
15
12
Manual
1 copy
5 copy
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