Diagnositc Kit for IgG Antibody to Hepatitis E Virus（ELISA）
Catalog No.: BE401A

PRINCIPLE

This kit employs solid phase, indirect ELISA method for detection of IgG antibodies to HEV (anti-HEV) in serum or plasma with two-step incubation procedure. Polystyrene microwell are pre-coated with purified activated recombinant HEV antigen. The HRP conjugated mouse anti human IgG (r chain) monoclonal antibody serves as tracer. TMB is substrate for HRP. The enzyme reaction with substrate TMB produces a color change, and the intensity of the absorbance at 450 nm indicates the presence or absence of Anti-HEV antibodies IgM in the sample.  The test is specific, sensitive, reproducible and easy to operate.  It is for blood screen of HEV infection.

MATERIALS PROVIDED

1. Antigen Coated Microwell Plate     1 block (96wells)
2. Specimen Diluent      1 bottle (12ml)
3. Negative Control      1 vial (1ml)
4. Positive Control      1 vial  (1ml)
5.20 X Wash Buffer (dilution prior to use)   1 bottle (30ml)
6.Enzyme Conjugant (anti human IgG -HRP)     1 bottle (12ml)
7. Substrate A      1 bottle (6ml)
8. Substrate B      1 bottle (6ml)
9. Stop Solution(2M H2SO4)      1 bottle (6ml)
10. Plastic Bag    1 bag
11. Seal Paper     3 pieces
12. Manual         1 each