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Nasopharyngeal test reagent
EBV-VCA IgA Ab ELISA Kit
Catalog No.: BE501A
Enzyme immunoassay for the detection of IgA antibody to the viral capsid antigen (VCA) of Epstein-Barr Virus (EBV) .
PRINCIPLE OF THE ASSAY
The EBV-VCA IgA Ab ELISA Kit utilizes a detection system where microplate wells are coated with recombinant antigen corresponding to a highly antigenic segment EBV-VCA. Serum or plasma specimens, controls are added to the wells. During incubation, IgA antibodies specific for EBV-VCA present in the specimen will bind to the recombinant antigen fixed onto the microplate wells. The wells are washed to remove unbound materials, and anti-human IgA peroxidase conjugate will bind to the antigen-antibody complex and excess unbound enzyme conjugates are again removed by washing. The enzyme substrate, tetramethylbenzidine (TMB), is added upon incubation the substrate will be hydrolyzed by the bound enzyme and a blue or blue-green colour develops in wells containing EBV-VCA IgA antibodies. The enzyme reaction is stopped by the addition of sulphuric acid. The intensity of colour developed is read spectrophotometrically at 450nm (450 nm/630 nm) and is proportional to the amount of antibodies present in the specimen.
REAGENTS
Materials provided with the kits:
|
Item |
Description |
96T |
480T |
|
1 |
Microtiter Well |
1 |
5 |
|
2 |
Negative Control |
1ml |
5ml |
|
3 |
Positive Control |
1ml |
5ml |
|
4 |
Sample Dilution |
12ml |
60ml |
|
5 |
Enzyme Conjugate |
12ml |
60ml |
|
6 |
Wash Buffer Concentrate (20x) |
30ml |
150ml |
|
7 |
Substrate Solution A |
6ml |
30ml |
|
8 |
Substrate Solution B |
6ml |
30ml |
|
9 |
Stop Solution |
6ml |
30ml |
|
10 |
Plastic Bag |
1 |
5 |
|
11 |
Seal Paper |
3 |
15 |
|
12 |
Manual |
1 copy |
5 copy |
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