Nasopharyngeal test reagent
- Product description
EBV-VCA IgA Ab ELISA Kit
Catalog No.: BE501A
Enzyme immunoassay for the detection of IgA antibody to the viral capsid antigen (VCA) of Epstein-Barr Virus (EBV) .PRINCIPLE OF THE ASSAY
The EBV-VCA IgA Ab ELISA Kit utilizes a detection system where microplate wells are coated with recombinant antigen corresponding to a highly antigenic segment EBV-VCA. Serum or plasma specimens, controls are added to the wells. During incubation, IgA antibodies specific for EBV-VCA present in the specimen will bind to the recombinant antigen fixed onto the microplate wells. The wells are washed to remove unbound materials, and anti-human IgA peroxidase conjugate will bind to the antigen-antibody complex and excess unbound enzyme conjugates are again removed by washing. The enzyme substrate, tetramethylbenzidine (TMB), is added upon incubation the substrate will be hydrolyzed by the bound enzyme and a blue or blue-green colour develops in wells containing EBV-VCA IgA antibodies. The enzyme reaction is stopped by the addition of sulphuric acid. The intensity of colour developed is read spectrophotometrically at 450nm (450 nm/630 nm) and is proportional to the amount of antibodies present in the specimen.
REAGENTS
Materials provided with the kits:
Item
Description
96T
480T
1
Microtiter Well
1
5
2
Negative Control
1ml
5ml
3
Positive Control
1ml
5ml
4
Sample Dilution
12ml
60ml
5
Enzyme Conjugate
12ml
60ml
6
Wash Buffer Concentrate (20x)
30ml
150ml
7
Substrate Solution A
6ml
30ml
8
Substrate Solution B
6ml
30ml
9
Stop Solution
6ml
30ml
10
Plastic Bag
1
5
11
Seal Paper
3
15
12
Manual
1 copy
5 copy
Search for

Tel : 86-10-60216750
Tel/Wechat/Whatsapp : 0086-18600464506
Email: Steven@bioneovan.com
Add:27 Yongwang Road, Daxing Biomedical Industry Base, Zhongguancun Science Park, Daxing District, Beijing
Message
Bioneovan Co.,Ltd 京ICP备11025455号-1 京公网安备 11011502003229号 Powered by 300.cn