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HCV Elisa Kit
HCV Ab ELISA Kit
Enzyme immunoassay for the detection of antibodies against Human Hepatitis Virus Type C (HCV)
PRINCIPLE OF THE ASSAY
Multiple epitopes of HCV proteins(Core, NS3, NS4 and NS5) are bound to the microtiter wells. When antibodies to HCV are present in the test sample, they react with recombinant proteins and attach to the solid-phase. Non-reactive antibodies are removed with the wash buffer. Human IgGs bound to the antigen are reacted with anti-human IgG peroxidase conjugate and visualized by subsequent reactions with a chromogenic substrate. Positive sample generates a medium to dark blue color. No color or very pale blue color indicates a negative reaction. The intensity of the reaction is photometrically quantitated.
REAGENTS
Materials provided with the kits:
|
Item |
Description |
96T |
480T |
|
1 |
Coating Plate |
1 |
5 |
|
2 |
Negative Control |
1ml |
5ml |
|
3 |
Positive Control |
1ml |
5ml |
|
4 |
Sample Diluent |
12ml |
60ml |
|
5 |
Enzyme Conjugate |
12ml |
60ml |
|
6 |
Wash Buffer Concentrate (20x) |
30ml |
150ml |
|
7 |
Substrate Solution A |
6ml |
30ml |
|
8 |
Substrate Solution B |
6ml |
30ml |
|
9 |
Stop Solution |
6ml |
30ml |
|
10 |
Plastic Bag |
1 |
5 |
|
11 |
Seal Paper |
3 |
15 |
|
12 |
Manual |
1 copy |
5 copy |
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