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HCV Elisa Kit

HCV

HCV

xx07

xx07

  • Product description
  • HCV Ab ELISA Kit
    Enzyme immunoassay for the detection of antibodies against Human Hepatitis Virus Type C (HCV)

    PRINCIPLE OF THE ASSAY 
    Multiple epitopes of HCV proteins(Core, NS3, NS4 and NS5) are bound to the microtiter wells.  When antibodies to HCV are present in the test sample, they react with recombinant proteins and attach to the solid-phase.  Non-reactive antibodies are removed with the wash buffer.  Human IgGs bound to the antigen are reacted with anti-human IgG peroxidase conjugate and visualized by subsequent reactions with a chromogenic substrate.  Positive sample generates a medium to dark blue color.  No color or very pale blue color indicates a negative reaction.  The intensity of the reaction is photometrically quantitated.
    REAGENTS
    Materials provided with the kits:

    Item

    Description

    96T

    480T

    1

    Coating  Plate

    1

    5

    2

    Negative Control

    1ml

    5ml

    3

    Positive Control

    1ml

    5ml

    4

    Sample Diluent

    12ml

    60ml

    5

    Enzyme Conjugate

    12ml

    60ml

    6

    Wash Buffer Concentrate (20x)

    30ml

    150ml

    7

    Substrate Solution A

    6ml

    30ml

    8

    Substrate Solution B

    6ml

    30ml

    9

    Stop Solution

    6ml

    30ml

    10

    Plastic Bag

    1

    5

    11

    Seal  Paper

    3

    15

    12

    Manual

    1 copy

    5 copy

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