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Nasopharyngeal test reagent

Nasopharyngeal test reagent

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  • Product description
  • EBV-VCA IgA  Ab ELISA Kit
    Catalog No.: BE501A

    Enzyme immunoassay for the detection of IgA antibody to the viral capsid antigen (VCA) of Epstein-Barr Virus  (EBV) .

    PRINCIPLE OF THE ASSAY

    The EBV-VCA IgA  Ab ELISA Kit utilizes a detection system where microplate wells are coated with recombinant antigen corresponding to a highly antigenic segment EBV-VCA. Serum or plasma specimens, controls are added to the wells. During incubation, IgA antibodies specific for EBV-VCA present in the specimen will bind to the recombinant antigen fixed onto the microplate wells.  The wells are washed to remove unbound materials, and anti-human IgA peroxidase conjugate will bind to the antigen-antibody complex and excess unbound enzyme conjugates are again removed by washing. The enzyme substrate, tetramethylbenzidine (TMB), is added upon incubation the substrate will be hydrolyzed by the bound enzyme and a blue or blue-green colour develops in wells containing EBV-VCA IgA antibodies.  The enzyme reaction is stopped by the addition of sulphuric acid.  The intensity of colour developed is read spectrophotometrically at 450nm (450 nm/630 nm) and is proportional to the amount of antibodies present in the specimen.

    REAGENTS

    Materials provided with the kits:

    Item

    Description

    96T

    480T

    1

    Microtiter Well

    1

    5

    2

    Negative Control

    1ml

    5ml

    3

    Positive Control

    1ml

    5ml

    4

    Sample Dilution

    12ml

    60ml

    5

    Enzyme Conjugate

    12ml

    60ml

    6

    Wash Buffer Concentrate (20x)

    30ml

    150ml

    7

    Substrate Solution A

    6ml

    30ml

    8

    Substrate Solution B

    6ml

    30ml

    9

    Stop Solution

    6ml

    30ml

    10

    Plastic Bag

    1

    5

    11

    Seal  Paper

    3

    15

    12

    Manual

    1 copy

    5 copy

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